FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\E2f1UAS.b.Tag:FLAG,Tag:MYC
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General Information
Symbol
Dmel\E2f1UAS.b.Tag:FLAG,Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0343973
Feature type
allele
Associated gene
Dmel\E2f1
Associated Insertion(s)
Carried in Construct
P{UAS-FM-E2f1.b}
Key Links
Transgenic product class
wild_type
(Kim et al., 2018)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kim et al., 2018)
Mutagen
in vitro construct
(Kim et al., 2018)
Progenitor genotype
Carried in construct
P{UAS-FM-E2f1.b}
Cytology
Description

UAS regulatory sequences drive expression of the 'b' isoform of E2f1, tagged at the N-terminal end with Tag:FLAG and Tag:MYC.

(Kim et al., 2018)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
E2f1
Tagged with
Tag:FLAG
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of E2f1UAS.b.Tag:FLAG,Tag:MYC under the control of Scer\GAL4GMR.PU leads to a disorganized compound eye and induces cell death (Dcp1-positive cells) and cell division (EdU-positive cells) in the eye disc.

      (Kim et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      E2f1UAS.a.Tag:FLAG,Tag:MYC, Scer\GAL4Ubi.PU, E2f1UAS.b.Tag:FLAG,Tag:MYC partially rescues Df(3R)Exel6186/E2f1rM729

      (Kim et al., 2018)

      Scer\GAL4Ubi.PU/E2f1UAS.b.Tag:FLAG,Tag:MYC partially rescues Df(3R)Exel6186/E2f1rM729

      (Kim et al., 2018)
      Comments

      The early larval lethality of E2f1rM729/Df(3R)Exel6186 transheterozygotes is partially rescues by Scer\GAL4Ubi.PU-driven expression of either E2f1UAS.a.Tag:FLAG,Tag:MYC or E2f1UAS.b.Tag:FLAG,Tag:MYC (giving rise to few, severely developmentally delayed third instar larvae and pupae, but no pharate adults), or of both transgenes (giving rise to the expected numbers of mildly developmentally delayed third instar larvae, pupae and to pharate adults, but to no adults).

      (Kim et al., 2018)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      E2f1UAS.b.Tag:FLAG,Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (1)