FlyBase Allele Report: Dmel\lola[F.stop]

General Information

Symbol

Dmel\lola^F.stop

Species

D. melanogaster

Name

FlyBase ID

FBal0344525

Feature type

allele

Associated gene

Dmel\lola

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Dinges et al., 2017)

Mutagen

CRISPR/Cas9

(Dinges et al., 2017)

Progenitor genotype

Cytology

Description

2bp deletion in the transcript encoding the lola-F isoform, resulting in a premature stop codon downstream of the zinc-finger domain.

(Dinges et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Dinges et al., 2017)

lethal - all die during embryonic stage

(Dinges et al., 2017)

Phenotype Manifest In

axon | embryonic stage

(Dinges et al., 2017)

embryonic/larval neuromuscular junction | embryonic stage

(Dinges et al., 2017)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage

(Dinges et al., 2017)

ventral midline of embryo | embryonic stage

(Dinges et al., 2017)

Detailed Description

Statement

Reference

In lola^F.stop stage 15/16 embryos ISNb motor nerves fail to form neuromuscular junctions and ventral nerve cord axons extend slightly further than controls and show crossing defects; the ventral nerve cord in stage 13/14 embryos show a disrupted arrangement of glial cells, without any changes in the numbers of glial cells, neurons and neuroblasts, as compared to controls.

(Dinges et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

lola^F.stop has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/futsch^EP1419

(Dinges et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

lola^F.stop has embryonic/larval neuromuscular junction | embryonic stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/futsch^EP1419

(Dinges et al., 2017)

lola^F.stop has axon | embryonic stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/futsch^EP1419

(Dinges et al., 2017)

lola^F.stop has ventral midline of embryo | embryonic stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/futsch^EP1419

(Dinges et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

lola^F.stop is rescued by Scer\GAL4^elav-C155/lola^{UASp.F.Tag:FLAG}

(Dinges et al., 2017)

Not rescued by

lola^F.stop is not rescued by Scer\GAL4^elav-C155/lola^{UASp.H.Tag:FLAG}

(Dinges et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

lola-F^Stop

(Dinges et al., 2017)

lola^F.stop

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close