Re-integration of mutated Khc (β5 loop 8 sequence, KIRDLLDVSKVNLSVHEDKNRVPYVKGATERFVSS has been substituted with the unc-104 loop β5 loop 8 sequence, RVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTD) into the attP site present in KhcKO.attP.
lethal (with Khc27)
majority die during second instar larval stage
partially lethal - majority die