FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\nenyaRNAi.UAS
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General Information
Symbol
Dmel\nenyaRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0345529
Feature type
allele
Associated gene
Dmel\nenya
Associated Insertion(s)
Carried in Construct
P{UAS-nenya.RNAi}
Also Known As
nenyaRNAi
Key Links
Transgenic product class
RNAi_reagent
(Lake et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Lake et al., 2019)
Mutagen
in vitro construct
(Lake et al., 2019)
Progenitor genotype
Carried in construct
P{UAS-nenya.RNAi}
Cytology
Description

UAS sequences (from the pValium22 vector) regulate expression of an RNAi hairpin (GGACATAGATTGCCTTGAAGA) that targets the nenya transcript for degradation.

(Lake et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
nenya
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Females expressing nenyadsRNA.UAS under the control of Scer\GAL4VP16.nos.UTR do not show significant meiotic chromosome segregation errors, as assessed by X-chromosome non-disjunction; in agreement, they do not show any obvious defect in meiotic recombination initiation in early pachytene cysts, as the expected DSBs are still detected (i.e. γH2AV foci).

      (Lake et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      naryaJJ6 homozygous females that also express nenyadsRNA.UAS under the control of Scer\GAL4VP16.nos.UTR show frequent meiotic chromosome segregation errors, as assessed by X-chromosome non-disjunction; in agreement, meiotic recombination fails to initiate in early pachytene cysts, as few/no DSBs (i.e. γH2AV foci) and no crossovers along the X chromosome are detected. The oocyte is correctly specified by early-mid pachytene but does not show any obvious defects in karyosome structure. These females do not show changes in fertility, as compared to controls.

      naryaG4 homozygous females that also express nenyadsRNA.UAS under the control of Scer\GAL4VP16.nos.UTR show frequent meiotic chromosome segregation errors, as assessed by X-chromosome non-disjunction. Although there are no obvious defects in meiotic recombination initiation in early pachytene cysts, as the frequent DSBs are still formed (i.e. γH2AV foci), this does not translate into crossovers along the X chromosome; however, this does not seem to be due to a global DSB repair defect, as there is no delay in the removal of γH2AV foci at mid-pachytene. The oocyte is correctly specified by early-mid pachytene but does not show any obvious defects in karyosome structure.

      naryaGFP/naryaGFP, Scer\GAL4VP16.nos.UTR>nenyadsRNA.UAS females do not show significant meiotic chromosome segregation errors, as assessed by X-chromosome non-disjunction.

      (Lake et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      nenyaRNAi.UAS
      nenyadsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)