The expression of aurB^G213D.UAS under the control of Scer\GAL4^sd.PU induces a severe reduction in wing size, as compared to controls.

Unlike control wing disc clones, wing disc clones expressing aurB^G213D.UAS under the control of Scer\GAL4^Act5C.PI fail to proliferate/expand, remaining single cells with single large and apparently polyploid nuclei.

The expression of aurB^G213D.UAS only before the binucleation stage (i.e. expression under the combined control of Scer\GAL4^NP5399 and Gal80[ts], and shift from the permissive 19[o]C temperature to the restrictive 29[o]C temperature only during 25h-60h after puparium formation) leads to significantly smaller male accessory gland lobes in the expanded state, as well as a significantly lower mating-induced shrinkage rate and shrinkage efficiency (i.e. ratio of estimated shrinkage:total ploidy per lobe), as compared to control. These male accessory gland lobes also exhibit significantly fewer cells and significantly lower overall ploidy than controls; moreover, whereas control male accessory gland cells are typically binucleate octoploid cells (4N x 2), aurB^G213D.UAS-expressing glands show a high proportion of tetraploid single-nucleated cells (4N x 1), some octoploid single-nucleated cells (4N x 1) and some cells with micro nucleus. This seem to correlate with reduced ejection of seminal fluid, as there is a significant decrease in accessory gland proteins (i.e. Sp-GFP) being transferred during mating, as compared to controls. Males expressing aurB^G213D.UAS induce lower egg laying in mated females 10-14 after mating, but not before or after that period.