FlyBase Allele Report: Dmel\heca[UAS.cLa]

General Information

Symbol

Dmel\heca^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Li

FlyBase ID

FBal0346094

Feature type

allele

Associated gene

Dmel\heca

Associated Insertion(s)

Carried in Construct

M{UAS-heca.L}

Key Links

Transgenic product class

cDNA

(Li et al., 2019)

wild_type

(Li et al., 2019)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Li et al., 2019)

wild_type

(Li et al., 2019)

Mutagen

in vitro construct

(Li et al., 2019)

Progenitor genotype

Carried in construct

M{UAS-heca.L}

Cytology

Description

UASt regulatory sequences drive expression of full-length hdc cDNA (LD44381).

(Li et al., 2019)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

heca

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^en.PU

(Li et al., 2019)

abnormal size | adult stage, with Scer\GAL4^GMR.PU

(Li et al., 2019)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Li et al., 2019)

decreased cell number | adult stage, with Scer\GAL4^nub.PU

(Li et al., 2019)

increased occurrence of cell division | larval stage, with Scer\GAL4^en.PU

(Li et al., 2019)

visible | adult stage, with Scer\GAL4^en.PU

(Li et al., 2019)

visible | adult stage, with Scer\GAL4^GMR.PU

(Li et al., 2019)

visible | adult stage, with Scer\GAL4^nub.PU

(Li et al., 2019)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Li et al., 2019)

wing, with Scer\GAL4^nub.PU

(Li et al., 2019)

wing disc posterior compartment, with Scer\GAL4^en.PU

(Li et al., 2019)

wing hair, with Scer\GAL4^nub.PU

(Li et al., 2019)

wing posterior compartment, with Scer\GAL4^en.PU

(Li et al., 2019)

Detailed Description

Statement

Reference

Expressing hdc^UAS.cLa under the control of either Scer\GAL4^GMR.PU or Scer\GAL4^nub.PU induces smaller eyes or wings, respectively, than in controls; these wings exhibit significantly fewer but denser trichomes (as quantified between L3 and L4) than controls.

Expressing hdc^UAS.cLa under the control of Scer\GAL4^GMR.PU does not induce ectopic cell apoptosis (in TUNEL assay) in the wing disc.

Expressing hdc^UAS.cLa under the control of Scer\GAL4^en.PU leads to more cells undergoing S-phase and mitosis in the posterior compartment of the wing disc (where Scer\GAL4^en.PU is driving expression), despite a significant decrease in wing compartment size, as compared to controls.

Clonal expression of hdc^UAS.cLa under the control of Scer\GAL4^Act.PU in the eye disc induces a cell cycle progression delay, as mutant second mitotic wave cells are less anterior than neighboring wild-type controls.

(Li et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, heca^UAS.cLa has abnormal size | adult stage phenotype, enhanceable by unk^UAS.cLa/Scer\GAL4^GMR.PU

(Li et al., 2019)

Scer\GAL4^GMR.PU, heca^UAS.cLa has visible | adult stage phenotype, enhanceable by unk^UAS.cLa/Scer\GAL4^GMR.PU