FlyBase Allele Report: Dmel\tplus3a[Δ]

General Information

Symbol

Dmel\tplus3a^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0347487

Feature type

allele

Associated gene

Dmel\tplus3a

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Hundertmark et al., 2019)

Mutagen

CRISPR/Cas9

(Hundertmark et al., 2019)

Progenitor genotype

Cytology

Description

A deletion of the tplus3a-tplus3b region leading to a fusion of tplus3a within its ORF to CR31700, an inactive pseudogene approximately 4,000 base pairs (bp) upstream, and yielding a null allele of tplus3a.

(Hundertmark et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

tplus3a^Δ, tplus3b^Δ has male sterile phenotype, suppressible by tplus3b^EGFP

(Hundertmark et al., 2019)

Other

Statement

Reference

tplus3a^Δ, tplus3b^Δ has male sterile phenotype

(Hundertmark et al., 2019)

tplus3a^Δ, tplus3b^Δ/Df(2L)BSC151 has male semi-fertile phenotype

(Hundertmark et al., 2019)

tplus3a^Δ, tplus3b^Δ/Df(2L)BSC151 has abnormal size | male | adult stage phenotype

(Hundertmark et al., 2019)

tplus3a^Δ, tplus3b^Δ/Df(2L)BSC151 has abnormal cell number | spermatogenesis phenotype

(Hundertmark et al., 2019)

Phenotype Manifest In

Other

Statement

Reference

tplus3a^Δ, tplus3b^Δ/Df(2L)BSC151 has seminal vesicle phenotype

(Hundertmark et al., 2019)

tplus3a^Δ, tplus3b^Δ/Df(2L)BSC151 has spermatozoon phenotype

(Hundertmark et al., 2019)

tplus3a^Δ, tplus3b^Δ/Df(2L)BSC151 has spermatid cyst phenotype

(Hundertmark et al., 2019)

tplus3a^Δ, tplus3b^Δ/Df(2L)BSC151 has individualization stage spermatid phenotype

(Hundertmark et al., 2019)

tplus3a^Δ, tplus3b^Δ/Df(2L)BSC151 has sperm individualization complex phenotype

(Hundertmark et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

tplus3a^Δ, tplus3b^Δ double homozygotes are male sterile; this phenotype is rescued by tplus3b^EGFP.

In the testes of tplus3a, tplus3b double mutants (tplus3a^Δ and tplus3b^Δ over Df(2L)BSC151) many spermatid bundles are arranged in parallel, but some are dispersed in the testis and lose their cyst organization. In some cases the individualization complex is abnormal in that one end is thickened. Although the seminal vesicles are empty of mature sperm in 1-day old males, they accumulate more mature sperm than controls in 5- and 6-days old males. In agreement, these males produce much fewer progeny than controls by day 4, but progressively reach the fertility of wild-type, Df(2L)BSC151 heterozygous, or tplus3a^Δ, tplus3b^Δ double heterozygous controls.

(Hundertmark et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

A single guide RNA was used to generate deletions in the related genes tplus3a and tplus3b, resulting in large parts of the open reading frame (ORF) of both genes being deleted.

(Hundertmark et al., 2019)

Separable from: tplus3b[Δ].

(Hundertmark et al., 2019)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tplus3a^Δ

Δtplus3a-tplus3b

(Hundertmark et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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