A αTub84B enhancer drives expression of Cas9 that is tagged at the N-terminal end with Tag:NLS(SV40-largeT) and at the C-terminal end with Tag:NLS(Npm-Unk).
The αTub84B enhancer drives expression of Cas9.
lethal - all die during second instar larval stage | parental effect, with RNaseZU6.3xsgRNA