FlyBase Allele Report: Dmel\bsk[gRNA.pCFD3-3]

General Information

Symbol

Dmel\bsk^gRNA.pCFD3-3

Species

D. melanogaster

Name

FlyBase ID

FBal0350173

Feature type

allele

Associated gene

Dmel\bsk

Associated Insertion(s)

Carried in Construct

M{U6:3-bsk.gRNA.pCFD3-3}

Key Links

Transgenic product class

sgRNA

(Meltzer et al., 2019)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

sgRNA

(Meltzer et al., 2019)

Mutagen

in vitro construct

(Meltzer et al., 2019)

Progenitor genotype

Carried in construct

M{U6:3-bsk.gRNA.pCFD3-3}

Cytology

Description

The ubiquitous snRNA:U6:96Ac promoter drives expression of a single sgRNA (GCACCAACACTACACCGTCG) that targets bsk.

(Meltzer et al., 2019)

Allele components

Component

Use(s)

Regulatory region(s)

snRNA:U6:96Ac

Encoded product / tool

bsk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

(Meltzer et al., 2019)

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(Meltzer et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Crispr-Cas9 mutagenesis of bsk by expressing either Spyo\Cas9^{UAS.Tag:NLS(Unk)} or Spyo\Cas9^UAS.P2 under the control of Scer\GAL4^GMR71G10 and using bsk^gRNA.pCFD3-3 as a guide, results in frequent mushroom body gamma neuron pruning defects; phenotype is milder when expressing Spyo\Cas9^UAS.P2.

(Meltzer et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

bsk^gRNA.pCFD3-3

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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