FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\uspgRNA.pCFD3-2
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General Information
Symbol
Dmel\uspgRNA.pCFD3-2
Species
D. melanogaster
Name
FlyBase ID
FBal0350193
Feature type
allele
Associated gene
Dmel\usp
Associated Insertion(s)
Carried in Construct
M{U6:3-usp.gRNA.pCFD3-2}
Key Links
Transgenic product class
sgRNA
(Meltzer et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
sgRNA
(Meltzer et al., 2019)
Mutagen
in vitro construct
(Meltzer et al., 2019)
Progenitor genotype
Carried in construct
M{U6:3-usp.gRNA.pCFD3-2}
Cytology
Description

The ubiquitous snRNA:U6:96Ac promoter drives expression of a single sgRNA (CTTTCGGCTGAGCCACATCA) that targets usp.

(Meltzer et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
usp
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Crispr-Cas9 mutagenesis of usp by expressing Spyo\Cas9UAS.Tag:NLS(Unk) under the control of Scer\GAL4GMR71G10 and using uspgRNA.pCFD3-2 as a guide, results in a small frequency of mushroom body gamma neuron pruning defects.

      (Meltzer et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      uspgRNA.pCFD3-2
      Name Synonyms
      Secondary FlyBase IDs
        References (1)