FlyBase Allele Report: Dmel\Nup160[RNAi.UAS.cUa]

General Information

Symbol

Dmel\Nup160^RNAi.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0352672

Feature type

allele

Associated gene

Dmel\Nup160

Associated Insertion(s)

Carried in Construct

P{UAS-Nup160.RNAi.U}

Key Links

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-Nup160.RNAi.U}

Cytology

Description

UAS regulates expression of an RNAi construct that targets Nup160.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nup160

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of nephrotic syndrome type 19

CEC

(Zhao et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: "nephrotic syndrome type 19" is associated with human ortholog NUP160.

(Zhao et al., 2019)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | larval stage, with Scer\GAL4^Ugt36A1.PK

(Zhao et al., 2019)

decreased cell number | adult stage, with Scer\GAL4^Ugt36A1.PK

(Zhao et al., 2019)

decreased cell number | larval stage | progressive, with Scer\GAL4^Ugt36A1.PK

(Zhao et al., 2019)

short lived, with Scer\GAL4^Ugt36A1.PK

(Zhao et al., 2019)

Phenotype Manifest In

nephrocyte | adult stage, with Scer\GAL4^Ugt36A1.PK

(Zhao et al., 2019)

nephrocyte | larval stage | progressive, with Scer\GAL4^Ugt36A1.PK

(Zhao et al., 2019)

nuclear lamina | larval stage, with Scer\GAL4^Ugt36A1.PK

(Zhao et al., 2019)

nuclear pore | larval stage, with Scer\GAL4^Ugt36A1.PK

(Zhao et al., 2019)

nucleus | larval stage, with Scer\GAL4^Ugt36A1.PK

(Zhao et al., 2019)

Detailed Description

Statement

Reference

Expression of Nup160^{dsRNA.UAS.cUa} under the control of Scer\GAL4^Ugt36A1.PK leads to a progressive decrease in the number of nephrocytes during development (from second instar to third instar larvae), and adults lack nephrocytes entirely. The larval nephrocytes are functionally impaired, as there is significantly lower RFP/Dextran uptake from hemolymph and significantly lower AgNO[[3]] sequestration by cardiac nephrocytes, as compared to controls; the larval nephrocytes also have reduced nuclear volume, as well as nuclear pore complex and nuclear lamin abnormalities.

(Zhao et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Nup160^RNAi.UAS.cUa, Scer\GAL4^Ugt36A1.PK has abnormal size | larval stage phenotype, suppressible by Hsap\NUP160^wt.UAS, Scer\GAL4^Ugt36A1.PK

(Zhao et al., 2019)

Nup160^RNAi.UAS.cUa, Scer\GAL4^Ugt36A1.PK has decreased cell number | adult stage phenotype, suppressible | partially by Hsap\NUP160^wt.UAS, Scer\GAL4^Ugt36A1.PK