FB2026_02 , released June 18, 2026
Allele: Avic\GFP20XUAS.SPARC2-D.Tag:M(mCd8a)
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General Information
Symbol
Avic\GFP20XUAS.SPARC2-D.Tag:M(mCd8a)
Species
A. victoria
Name
FlyBase ID
FBal0353087
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
TI{20XUAS-SPARC2-D-mCD8::GFP}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Yang and Isaacman-Beck, 2019.9.30)
Progenitor genotype
Carried in construct
TI{20XUAS-SPARC2-D-mCD8::GFP}
Cytology
Description

20XUAS regulatory sequences are separated from sequence encoding Tag:M(mCd8a)-tagged GFP by a "SPARC2-D" module that comprises: a full-length attP site of 60bp, a STOP cassette, a second attP site (60bp, full-length site), ribozyme sequence (to prevent leaky expression in the absence of phiC31:int) and an attB site. phiC31:int catalyzes irreversible recombination between the attB site and one of the two attP sites; if the attP site upstream of the STOP cassette is utilized, the STOP cassette is excised, permitting expression of Tag:M(mCd8a)-tagged GFP, while if the attP site downstream of the STOP cassette is utilized, the STOP cassette is preserved. The "SPARC2-D" module typically results in expression of the effector (in this case Tag:M(mCd8a)-tagged GFP) in approximately 45% of cells.

(Yang and Isaacman-Beck, 2019.9.30)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
GFP
Tagged with
Tag:M(mCd8a)
Also carries
attB
attP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Avic\GFP20XUAS.SPARC2-D.Tag:M(mCd8a)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)