Germline specific mutagenesis of Trpm, by using Trpm^U6:3.gRNA1 as sgRNA source together with Spyo\Cas9^nos.PU, results in a significant proportion of females exhibiting rudimentary ovaries. Where ovaries have grossly normal morphology, there is a lower incidence of calcium waves upon in vitro egg activation, as compared to controls. Egg activation still occur in vivo as: unfertilized eggs and embryos are resistant to 50% bleach, which is indicative of vitelline membrane cross-linking; unfertilized embryos still show polar body formation; and early embryos undergo normal mitosis; although fewer larvae hatch.

Germline specific mutagenesis of Trpm, by using Trpm^U6:3.gRNA1 as sgRNA source and using Scer\GAL4^{VP16.mat.αTub67C} to drive Spyo\Cas9^{UAS.Tag:NLS(Unk)} does not grossly affect ovary morphology. However, there is a much lower incidence of calcium waves upon in vitro egg activation, as compared to controls. Egg activation still occur in vivo as: unfertilized eggs and embryos are resistant to 50% bleach, which is indicative of vitelline membrane cross-linking; and early embryos undergo normal mitosis; although fewer larvae hatch.