FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Rnor\Apobec1Tag:Mito(hATP5F1B),Tag:HA
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General Information
Symbol
Rnor\Apobec1Tag:Mito(hATP5F1B),Tag:HA
Species
R. norvegicus
Name
FlyBase ID
FBal0356082
Feature type
allele
Associated gene
Rnor\Apobec1
Associated Insertion(s)
Carried in Construct
P{mito-APOBEC1}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Andreazza et al., 2019)
Progenitor genotype
Carried in construct
P{mito-APOBEC1}
Cytology
Description

UASt regulates expression of a "mtDNA mutator" construct based on a mitochondrially-targeted cytidine deaminase, APOBEC1. The Rnor\Apobec1 cDNA is placed downstream of the mitochondrial targeting sequence of human ATP synthase subunit F1β (Tag:Mito(hATP5F1B)), a Tag:HA epitope and a flexible linker sequence 5'. A nuclear exporting sequence (NES) is inserted 3' to Rnor\Apobec1.

(Andreazza et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rnor\Apobec1
Tagged with
Tag:HA
Tag:Mito(hATP5F1B)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Rnor\Apobec1Tag:Mito(hATP5F1B),Tag:HA under the control of Scer\GAL4da.G32 leads to an impaired climbing ability and a shortened lifespan in young flies when compared to controls.

      (Lee et al., 2020)

      The expression of Rnor\Apobec1Tag:Mito(hATP5F1B),Tag:HA under the control of Scer\GAL4da.G32 does not induce any gross development or viability defect, but drastically shortens lifespan and climbing ability. Adults show higher levels of mitochondrial DNA (mtDNA) mutations, although indels and larger deletions are rare, there is no/minimal impact on mtDNA levels or mtDNA integrity; almost all mutations are C:G > T:A transitions; most of the cytidine deamination events occur in the minor strand; the frequency of mutations does not change with age; there is a significantly higher NS:S mutation ratio and mutations disproportionally target the 1st and 2nd codons in protein-coding genes, as compared to controls.

      (Andreazza et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      In young flies, StingΔRG5 and park25 mutations together enhance the impaired climbing ability phenotype observed as a result of expression of Rnor\Apobec1Tag:Mito(hATP5F1B),Tag:HA under the control of Scer\GAL4da.G32, while StingΔRG5 or park25 mutation alone does not modify that phenotype.

      (Lee et al., 2020)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Rnor\Apobec1Tag:Mito(hATP5F1B),Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)