FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\rlE335K.UAS.cTa
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General Information
Symbol
Dmel\rlE335K.UAS.cTa
Species
D. melanogaster
Name
FlyBase ID
FBal0356139
Feature type
allele
Associated gene
Dmel\rl
Associated Insertion(s)
Carried in Construct
P{UAS-rl.E335K.T}
Key Links
Genomic Maps

Transgenic product class
missense_variant
(Taylor et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Taylor et al., 2019)
Mutagen
in vitro construct
(Taylor et al., 2019)
Progenitor genotype
Carried in construct
P{UAS-rl.E335K.T}
Cytology
Description

UAS regulatory sequences drive expression of rl that has been mutated to carry a E335K amino acid replacement. This equivalent to a E322K change in the human MAPK1 gene, a change that is the most frequent ERK2 mutation found in cancers.

(Taylor et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
rl
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:1,113,723..1,113,723 [+]
Nucleotide change:

G1113723A

Amino acid change:

E335K | rl-PC; E335K | rl-PD; E335K | rl-PE; E290K | rl-PG; E225K | rl-PH; E335K | rl-PI

Reported amino acid change:

E335K

Comment:

Analogous E322K mutation in human MAPK1 implicated in cancer; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.

(Taylor et al., 2019)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      DOES NOT model  cancer
      CEA
      (Taylor et al., 2019)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Taylor et al., 2019)
      MAPK1:p.Glu322Lys
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: MAPK1_376450
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      rlE335K.UAS.cTa
      Name Synonyms
      Secondary FlyBase IDs
        References (2)