FB2025_01 , released February 20, 2025
Allele: Dmel\cystR.S320E.N-sfGFP
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General Information
Symbol
Dmel\cystR.S320E.N-sfGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0356953
Feature type
allele
Associated gene
Dmel\cyst
Associated Insertion(s)
Carried in Construct
P{sfGFP-cyst.R.S320E}
Key Links
Transgenic product class
missense_variant
(Silver et al., 2019)
polypeptide_post_translational_processing_variant
(Silver et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Silver et al., 2019)
polypeptide_post_translational_processing_variant
(Silver et al., 2019)
Mutagen
in vitro construct
(Silver et al., 2019)
Progenitor genotype
Carried in construct
P{sfGFP-cyst.R.S320E}
Cytology
Description

Genomic fragment that encompasses the cyst gene and includes its promoter (derived from the BACR27M12 BACPAC clone). A S320E amino acid replacement (phosphomimetic mutation) has been introduced into the coding sequence. Silent mutations have been introduced at two distinct sites to confer resistance to RNAi mediated by the P{TRiP.HMS01750} transgene. A sfGFP tag followed by a S(GGGGS)[[2]] linker has been inserted at the N-terminal end of the coding sequence.

(Silver et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
cyst
Encoded product / tool
cyst
Tagged with
sfGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      cystR.S320E.N-sfGFP rescues cystHMS01750

      (Silver et al., 2019)
      Comments

      Maternal expression of cystHMS01750 under the control of Scer\GAL4VP16.mat.αTub67C leads to embryonic lethality, which can be rescued by cystR.S320E.N-sfGFP.

      (Silver et al., 2019)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      cystR.S320E.N-sfGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)