FB2026_02 , released June 18, 2026
Recombinant Construct: P{sfGFP-cyst.R.S320E}
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General Information
Symbol
P{sfGFP-cyst.R.S320E}
FlyBase ID
FBtp0140653
Feature type
transgenic_transposable_element
Component Allele(s)
cystR.S320E.N-sfGFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele cystR.S320E.N-sfGFP
Transgenic product class
missense_variant
(Silver et al., 2019)
polypeptide_post_translational_processing_variant
(Silver et al., 2019)
Component
Use(s)
Regulatory region(s)
cyst
Encoded product / tool
cyst
Tagged with
sfGFP
Description

Genomic fragment that encompasses the cyst gene and includes its promoter (derived from the BACR27M12 BACPAC clone). A S320E amino acid replacement (phosphomimetic mutation) has been introduced into the coding sequence. Silent mutations have been introduced at two distinct sites to confer resistance to RNAi mediated by the P{TRiP.HMS01750} transgene. A sfGFP tag followed by a S(GGGGS)[[2]] linker has been inserted at the N-terminal end of the coding sequence.

(Silver et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
GFP::CystRS320E
(Silver et al., 2019)
P{sfGFP-cyst.R.S320E}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)