FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Uuuu\UbUAS.6.GG.Tag:polyHis
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General Information
Symbol
Uuuu\UbUAS.6.GG.Tag:polyHis
Species
u. unknown
Name
FlyBase ID
FBal0361970
Feature type
allele
Associated gene
Uuuu\Ub
Associated Insertion(s)
Carried in Construct
P{UAS-His6-Ub6-GG}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Blount et al., 2018)
Progenitor genotype
Carried in construct
P{UAS-His6-Ub6-GG}
Cytology
Description

UAS regulatory sequences (from pWalium10.moe) drive expression of an unanchored, uncleavable chain of six tandem copies of Ubiquitin; the chain lacks the internal "GG" motifs that are necessary for isopeptide bond formation and dismantling by deubiquitinases. The polyUbiquitin chain has a terminal "GG", meaning that it can potentially be conjugated onto other proteins; experiments in vivo show that this can occur in transgenic flies. The coding sequence is tagged at the N-terminal end with Tag:polyHis.

(Blount et al., 2018)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Uuuu\Ub
Tagged with
Tag:polyHis
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Uuuu\UbUAS.6.GG.Tag:polyHis
      Name Synonyms
      Secondary FlyBase IDs
        References (2)