FB2026_03 , released September 17, 2026
Allele: Dmel\kibraT2A-GFPnls
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General Information
Symbol
Dmel\kibraT2A-GFPnls
Species
D. melanogaster
Name
FlyBase ID
FBal0362384
Feature type
allele
Associated gene
Dmel\kibra
Associated Insertion(s)
TI{2A-EGFP}kibraT2A-GFPnls
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Albert et al., 2018)
Progenitor genotype
Mi{MIC}kibraMI13703
(Albert et al., 2018)
Associated Insertion(s)
TI{2A-EGFP}kibraT2A-GFPnls
Cytology
Description

The RMCE cassette in the Mi{MIC}kibraMI13703 insertion (which is in the kibra fourth intron upstream of exon 5) has been replaced by a sequence cassette consisting of kibra exons 5-9, a T2A site and EGFP tagged with a nuclear localization signal (Tag:NLS(Unk)). A loxP cassette containing a w+* marker has also been inserted, downstream of the T2A-EGFP-Tag:NLS(Unk) sequence. The inserted sequence is expected to be incorporated into the kibra mRNA, recapitulating a mRNA that encodes wild-type kibra protein, while the T2A sequence promotes the separate translation of the EGFP-Tag:NLS(Unk) reporter.

(Albert et al., 2018)
Allele components
Component
Use(s)
Inserted element
TI{2A-EGFP}
Encoded product / tool
EGFP
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      kibraT2A-GFPnls
      Name Synonyms
      Secondary FlyBase IDs
        References (1)