FlyBase Allele Report: Dmel\Alms1a[HMJ30289]

General Information

Symbol

Dmel\Alms1a^HMJ30289

Species

D. melanogaster

Name

FlyBase ID

FBal0363170

Feature type

allele

Associated gene

Dmel\Alms1a

Associated Insertion(s)

Carried in Construct

P{TRiP.HMJ30289}

Also Known As

UAS-alms1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Chen and Yamashita, 2020)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Chen and Yamashita, 2020)

Mutagen

in vitro construct

(Chen and Yamashita, 2020)

Progenitor genotype

Carried in construct

P{TRiP.HMJ30289}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Chen and Yamashita, 2020)

Note that P{TRiP.HMJ30289} is expected to knockdown both Alms1a and Alms1b due to the high degree of their sequence similarity.

(Chen and Yamashita, 2020)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMJ30289

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

centrosome | absent | male, with Scer\GAL4^{VP16.nanos.UTR}

(Chen and Yamashita, 2020)

male germline cell, with Scer\GAL4^{VP16.nanos.UTR}

(Chen and Yamashita, 2020)

Detailed Description

Statement

Reference

Expression of Alms1a^HMJ30289 under the control of Scer\GAL4^VP16.nos.UTR leads to centrosome loss in male germline cells except for the mother centrosomes in germline stem cells (which gradually elongate). Expression of Alms1a^HMJ30289 under the control of Scer\GAL4^VP16.nos.UTR in combination with tub-Gal80[ts] (to restrict expression to adult stages) leads to daughter centrosome loss in male germline stem cells as fast as 1-2 days.

(Chen and Yamashita, 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Alms1a^HMJ30289, Scer\GAL4^{VP16.nanos.UTR} has centrosome | male | absent phenotype, suppressible by upd1^UAS.cZa, Scer\GAL4^{VP16.nanos.UTR}

(Chen and Yamashita, 2020)