Sequence encoding the 5'UTR, coding sequence and 3'UTR of Wnt10, with a Tag:HA tag inserted in an unconserved region in exon 6, has been inserted into the attP site present in TI{TI}Wnt10KO.attP. This allows expression of the Tag:HA-tagged Wnt10 sequence under the control of endogenous Wnt10 regulatory sequences.