An Hsp70 promoter drives expression of a single-chain variable fragment antibody that binds both denatured and active Poxn protein (FBrf0105242).
Expression of Mus\scFvhs.Poxn.2.T:Hsap\MYC using heat shock in 4.5-5.5 hour old embryos can result in a reduction in the number of kolbchen. Expression of Mus\scFvhs.Poxn.2.T:Hsap\MYC using heat shock at 8 hours 20 minutes to 9 hours 20 minutes of development has no effect on the external morphology and number of kolbchen.