FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\bolM-introns.KO
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General Information
Symbol
Dmel\bolM-introns.KO
Species
D. melanogaster
Name
FlyBase ID
FBal0365225
Feature type
allele
Associated gene
Dmel\bol
Associated Insertion(s)
TI{TI}bolM-introns.KO
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Gao et al., 2020)
Progenitor genotype
TI{TI}bolKO.attP
(Gao et al., 2020)
Associated Insertion(s)
TI{TI}bolM-introns.KO
Cytology
Description

DNA has been re-integrated into the attP site in the TI{TI}bolKO.attP insertion, reconstituting a bol locus that is missing all introns between exon 2 and exon 7. An attR site is present upstream of exon 2 and a loxP site is present downstream of exon 8'. The two major circRNAs that are normally produced from the bol locus (circEx2-3 and circEx4-7) are absent in this mutant, while the levels of linear bol mRNA and bol protein are similar to wild type.

(Gao et al., 2020)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      bolM-introns.KO mature sperm (in the seminal vesicle) show abnormal nuclear morphology and are frequently apoptotic.

      (Gao et al., 2020)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      bolM-introns.KO
      Name Synonyms
      Secondary FlyBase IDs
        References (1)