FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\PMCAkisser.UAS.FAPα2
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General Information
Symbol
Dmel\PMCAkisser.UAS.FAPα2
Species
D. melanogaster
Name
FlyBase ID
FBal0365633
Feature type
allele
Associated gene
Dmel\PMCA
Associated Insertion(s)
Carried in Construct
P{UAS-PMCA-kisser-FAP}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Stawarski et al., 2018)
Progenitor genotype
Carried in construct
P{UAS-PMCA-kisser-FAP}
Cytology
Description

UAS regulatory sequences drive expression of a fusion protein composed of the Tag:SS(wg) signal peptide, a Tag:HA epitope, the FAPα2 fluorogen-activating peptide, a Tag:MYC epitope, a transmembrane domain (Tag:TM(hPDGFRB)) and a modified carboxy terminus of PMCA (the Cam binding site in the PMCA sequence has been scrambled to prevent unwanted oligomerization or cleavage by calpains). The configuration of the fusion protein results in the FAPα2 sequence and epitope tags being extracellular, while the PMCA sequence is intracellular (as in the endogenous PMCA protein).

(Stawarski et al., 2018)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
PMCA
Tagged with
FAPα2
Tag:HA
Tag:MYC
Tag:SS(wg)
Tag:TM(hPDGFRB)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      PMCAkisser.UAS.FAPα2
      Name Synonyms
      Secondary FlyBase IDs
        References (2)