FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Mnat9U6.gRNA.pCFD4
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General Information
Symbol
Dmel\Mnat9U6.gRNA.pCFD4
Species
D. melanogaster
Name
FlyBase ID
FBal0366578
Feature type
allele
Associated gene
Dmel\Mnat9
Associated Insertion(s)
Carried in Construct
P{U6-Mnat9.gRNA.pCFD4}
Key Links
Transgenic product class
sgRNA
(Mok and Choi, 2021)
Mutagen
Nature of the Allele
Transgenic product class
sgRNA
(Mok and Choi, 2021)
Mutagen
in vitro construct
(Mok and Choi, 2021)
Progenitor genotype
Carried in construct
P{U6-Mnat9.gRNA.pCFD4}
Cytology
Description

CRISPR transgene designed to result in deletion in a target gene when combined with a source of Cas9. The snRNA:U6:96Aa and snRNA:U6:96Ac promoters drive two gRNAs targeting the Mnat9 coding region.

(Mok and Choi, 2021)
Allele components
Component
Use(s)
Encoded product / tool
Mnat9
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Wing tissue-specific CRISPR mutagenesis of Mnat9 (by using Mnat9U6.gRNA.pCFD4 and expressing Spyo\Cas9UAS.P2 under the control of Scer\GAL4nub-AC-62) leads to wing notches.

      (Mok and Choi, 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Mnat9U6.gRNA.pCFD4
      Name Synonyms
      Secondary FlyBase IDs
        References (1)