A w+* marker replaces the region spanning around 540nt upstream of the ATG start codon of Tre1 up to the last 23 residues of the Tre1 ORF.
Approximate boundaries of a deletion that extends from about 540bp upstream of the Tre1 ATG to the last 23 residues of the Tre1 ORF. A A w+* marker is inserted at the site of the deletion.
primordial germ cell | embryonic stage (with Tre1sctt)
primordial germ cell | embryonic stage (with Tre1ΔEP5)
primordial germ cell | embryonic stage (with Tre1ΔEP19)
primordial germ cell | embryonic stage | maternal effect
PIP5K59BKD.UAS.Tag:HA, Scer\GAL4VP16.nanos.UTR, Tre1KO has primordial germ cell | embryonic stage phenotype
Tre1KO is rescued by Tre1UAS.Tag:FLAG/Scer\GAL4VP16.nanos.UTR
Tre1KO is rescued by Scer\GAL4VP16.nanos.UTR/Tre1UAS.Tag:HA
Tre1KO is rescued by Scer\GAL4VP16.nanos.UTR/Tre1UAS.cKa
Tre1KO is not rescued by Scer\GAL4VP16.nanos.UTR/Tre1UAS.EGFP