FB2026_03 , released September 17, 2026
Allele: Dmel\par-1KD.UASp.N1S.RR.EGFP
Open Close
General Information
Symbol
Dmel\par-1KD.UASp.N1S.RR.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0367129
Feature type
allele
Associated gene
Dmel\par-1
Associated Insertion(s)
Carried in Construct
P{UASp-EGFP.par-1.N1S.KD.RR}
Key Links
Transgenic product class
missense_variant
(Jiang and Harris, 2019)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Jiang and Harris, 2019)
Mutagen
in vitro construct
(Jiang and Harris, 2019)
Progenitor genotype
Carried in construct
P{UASp-EGFP.par-1.N1S.KD.RR}
Cytology
Description

UASp regulatory sequences drive expression of the par-1 N1S isoform (amplified from the RE47050 cDNA clone), mutated to produce a kinase-deficient form of the protein (amino acids T408 and S412 have been mutated). The coding sequence is tagged at the N-terminal end with EGFP. Silent mutations have been introduced into the coding sequence (TAGTTAAATTGTTCCAAGTAA to TCGTCAAGCTATTTCAGGTTA) to render the construct resistant to RNAi mediated by P{TRiP.GL00253}.

(Jiang and Harris, 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
par-1
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      par-1KD.UASp.N1S.RR.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)