FlyBase Allele Report: Dmel\LpR1[U6:3.2xgRNA.short]

General Information

Symbol

Dmel\LpR1^{U6:3.2xgRNA.short}

Species

D. melanogaster

Name

FlyBase ID

FBal0367725

Feature type

allele

Associated gene

Dmel\LpR1

Associated Insertion(s)

Carried in Construct

P{U6:3-LpR1.2xgRNA.short}

Key Links

Transgenic product class

sgRNA

(Yin et al., 2021)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

sgRNA

(Yin et al., 2021)

Mutagen

in vitro construct

(Yin et al., 2021)

Progenitor genotype

Carried in construct

P{U6:3-LpR1.2xgRNA.short}

Cytology

Description

The ubiquitous snRNA:U6:96Ac promoter drives expression of two sets of sgRNAs (GGAAAAAACTACGGAAAATG and TTTCGCGTTCTGGCTATAAA) targeting the short isoform-specific exon 6 of LpR1.

(Yin et al., 2021)

Allele components

Component

Use(s)

Regulatory region(s)

snRNA:U6:96Ac

Encoded product / tool

LpR1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^P2.4.Pdf, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(Yin et al., 2021)

partially lethal - majority die, with Spyo\Cas9^Act5C.cPa

(Yin et al., 2021)

Phenotype Manifest In

dendrite | larval stage, with Scer\GAL4^P2.4.Pdf, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(Yin et al., 2021)

larval brain | larval stage, with Scer\GAL4^P2.4.Pdf, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(Yin et al., 2021)

LNv neuron | larval stage, with Scer\GAL4^P2.4.Pdf, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(Yin et al., 2021)

Detailed Description

Statement

Reference

CRISPR/CAS9 mutagenesis of LpR1 long isoform in Pdf-positive neurons (LpR1^{U6:3.2xgRNA.short}, Scer\GAL4^P2.4.Pdf>Spyo\Cas9^{UAS.Tag:NLS(Unk)}) leads to LNv neurons in the larval brain to show decreased dendritic volume.

(Yin et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

LpR1^{U6:3.2xgRNA.short}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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