UASt regulatory sequences drive expression of α-Cat which is tagged at the N-terminal end (via a GSAGSAAGSGEF linker) with VHH(GFP4) and at the C-terminal end with TagRFP fluorescent protein.
α-CatUAS.VHH(GFP4),TagRFP, Scer\GAL4ap-md544, sqhEGFP.29B has leg disc | P-stage phenotype