The loxP cassette in TI{TI}rumpelΔ+Cherry has been excised, leaving a single attP site and a single loxP site in place of the rumpel gene (which has been completely deleted).
The entire rumpel gene has been deleted and replaced with an attP site, and a loxP cassette containing a Disc\RFPmCherry.3xP3 marker. Position of deletion endpoints on reference sequence determined from reported primer sequences used to generate homology arms.