FlyBase Allele Report: Dmel\Sarm[U6:3.gRNA]

General Information

Symbol

Dmel\Sarm^U6:3.gRNA

Species

D. melanogaster

Name

FlyBase ID

FBal0373145

Feature type

allele

Associated gene

Dmel\Sarm

Associated Insertion(s)

Carried in Construct

PBac{U6:3-Sarm.gRNA}

Key Links

Transgenic product class

sgRNA

(Ji et al., 2022)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

sgRNA

(Ji et al., 2022)

Mutagen

in vitro construct

(Ji et al., 2022)

Progenitor genotype

Carried in construct

PBac{U6:3-Sarm.gRNA}

Cytology

Description

A snRNA:U6:96Ac promoter drives expression of two sgRNAs (GCATCTGTTCAAACACTCCG and CGATTCCAATATCAGCCCGG), each of which target Sarm for knockout.

(Ji et al., 2022)

Allele components

Component

Use(s)

Regulatory region(s)

snRNA:U6:96Ac

Encoded product / tool

Sarm

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

abnormal stress response | larval stage, with Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Phenotype Manifest In

dendrite | larval stage, with Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

larval dorsal multidendritic neuron ddaC, with Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8 has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^ppk.PH/Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}/Cdc50^sgRNA.U6, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8 has abnormal stress response | larval stage phenotype, suppressible by Scer\GAL4^ppk.PH/Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}/Cdc50^sgRNA.U6, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Suppressor of

Statement

Reference

Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8 is a suppressor of decreased size | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Sarm^U6:3.gRNA, Spyo\Cas9^ppk.PP is a suppressor of abnormal neuroanatomy | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP

(Ji et al., 2022)

Sarm^U6:3.gRNA, Spyo\Cas9^ppk.PP is a suppressor of decreased size | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP

(Ji et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8 has dendrite | larval stage phenotype, suppressible by Scer\GAL4^ppk.PH/Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}/Cdc50^sgRNA.U6, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8 has larval dorsal multidendritic neuron ddaC phenotype, suppressible by Scer\GAL4^ppk.PH/Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}/Cdc50^sgRNA.U6, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Suppressor of

Statement

Reference

Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8 is a suppressor of dendrite | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8 is a suppressor of larval dorsal multidendritic neuron ddaC phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Sarm^U6:3.gRNA, Spyo\Cas9^ppk.PP is a suppressor of dendrite | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP

(Ji et al., 2022)

Sarm^U6:3.gRNA, Spyo\Cas9^ppk.PP is a suppressor of larval dorsal multidendritic neuron ddaC phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP

(Ji et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

606439

w^*; PBac{U6:3-Sarm.gRNA}VK00027

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Sarm^U6:3.gRNA

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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