FB2026_03 , released September 17, 2026
Allele: Hsap\RELAp65(AD)::pMagFast2.Ubi
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General Information
Symbol
Hsap\RELAp65(AD)::pMagFast2.Ubi
Species
H. sapiens
Name
FlyBase ID
FBal0374078
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{Ubi-p65(AD)::pMagFast2}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(di Pietro et al., 2021)
Progenitor genotype
Carried in construct
P{Ubi-p65(AD)::pMagFast2}
Cytology
Description

The Ubi-p63E promoter drives expression of the Hsap\RELA transcription activation domain fused to two copies of 'pMagFast2', a positively charged engineered domain that can heterodimerize with a compatible negatively charged 'nMag'-type domain upon stimulation with blue light (for details of pMagFast2 see PMID:25708714). This protein was designed to act as a hemidriver in the ShineGAL4 system, in which a transcription activation domain fused to a pMag-type domain can combine in vivo with a DNA-binding domain fused to a compatible nMag-type domain upon exposure to blue light, reconstituting a functional transcriptional activator in a light-dependent manner. However, when the Hsap\RELAp65(AD)::pMagFast2.Ubi protein was expressed together with the protein produced by Scer\GAL4DBD.nMagHigh1.Ubi in the presence of blue light, no expression of a reporter was detectable.

(di Pietro et al., 2021)
Allele components
Component
Use(s)
Regulatory region(s)
Ubi-p63E
Encoded product / tool
Hsap\RELA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Hsap\RELAp65(AD)::pMagFast2.Ubi
      Name Synonyms
      Secondary FlyBase IDs
        References (1)