20xUAS regulatory sequences drive expression of a bicistronic transcript that encodes two open reading frames separated by a T2A linker (this results in separate translation of each protein). The first ORF encodes iRFP713(V256C). The second ORF encodes a heme oxygenase 1 (HO1) gene that is required for iRFP713(V256C) to fluoresce.