FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Ecol\lacZKr.CD2swap.Tag:MS2
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General Information
Symbol
Ecol\lacZKr.CD2swap.Tag:MS2
Species
E. coli
Name
FlyBase ID
FBal0375194
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{Kr(CD2swap)-24xMS2-lacZ}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Scholes et al., 2019)
Progenitor genotype
Carried in construct
P{Kr(CD2swap)-24xMS2-lacZ}
Cytology
Description

An approximately 4.5kb Kr regulatory fragment from upstream of the Kr transcription start site is fused upstream of the Kr core promoter (sequence extends to the beginning of the second exon) followed by 24 copies of the Tag:MS2 stem loop and lacZ. The proximal (CD2) blastoderm enhancer normally present in the Kr regulatory fragment has been replaced with equivalent length of 'neutral' sequence and the distal (CD1) blastoderm enhancer sequence has been has been replaced with proximal (CD2) blastoderm enhancer sequence, resulting in a single copy of the CD2 enhancer in a non-endogenous location.

(Scholes et al., 2019)
Allele components
Component
Use(s)
Encoded product / tool
lacZ
Tagged with
Tag:MS2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ecol\lacZKr.CD2swap.Tag:MS2
      Name Synonyms
      Secondary FlyBase IDs
        References (1)