FB2026_02 , released June 18, 2026
Allele: Dmel\Src42AC.Y400D.UAS.N-VHH(dGBP1),Tag:HA
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General Information
Symbol
Dmel\Src42AC.Y400D.UAS.N-VHH(dGBP1),Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0387760
Feature type
allele
Associated gene
Dmel\Src42A
Associated Insertion(s)
Carried in Construct
M{UAS-dGBP1-HA::C-Src42A.Y400D}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Lepeta et al., 2022)
Progenitor genotype
Carried in construct
M{UAS-dGBP1-HA::C-Src42A.Y400D}
Cytology
Description

UASt regulatory sequences drive expression of a fusion protein composed of the destabilized VHH(dGBP1) nanobody, a Tag:HA tag and a truncated, constitutively active form of Src42A (amino acids 214-517, spanning the ATP-binding site and kinase domain, and containing two amino acid substitutions: Y511F (mutation in a crucial regulatory residue) and Y400D). The protein is designed to act as an engineered kinase; the VHH(dGBP1) nanobody should target the active kinase domain to proteins that are tagged with GFP (the target of the VHH(dGBP1) nanobody). In the absence of a GFP-tagged target, the nanobody is expected to be degraded.

(Lepeta et al., 2022)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Src42A
Tagged with
Tag:HA
VHH(dGBP1)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Src42AC.Y400D.UAS.N-VHH(dGBP1),Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)