FB2026_02 , released June 18, 2026
Allele: Mmus\Cd8asnRNA:U6:96Ac.4gRNAs
Open Close
General Information
Symbol
Mmus\Cd8asnRNA:U6:96Ac.4gRNAs
Species
M. musculus
Name
FlyBase ID
FBal0388251
Feature type
allele
Associated gene
Mmus\Cd8a
Associated Insertion(s)
Carried in Construct
P{U6-4gRNAs(mCD8)}
Transgenic product class
sgRNA
(Chen et al., 2020)
Mutagen
Nature of the Allele
Transgenic product class
sgRNA
(Chen et al., 2020)
Mutagen
in vitro construct
(Chen et al., 2020)
Progenitor genotype
Carried in construct
P{U6-4gRNAs(mCD8)}
Cytology
Description

The snRNA:U6:96Ac promoter is separated from four gRNAs (each with an attP site upstream of the gRNA) by an attB site and a Disc\RFP3xP3.PB marker. In the absence of phiC31:int, the Disc\RFP3xP3.PB marker prevents expression of the gRNAs. In the presence of phiC31:int, the attB site can recombine stochastically with any of the four attP sites upstream of a gRNA, removing the marker, creating an attR site and allowing expression of a single gRNA (the one immediately downstream of the attR site). Each of the gRNAs targets a different region of the Tag:M(mCd8a) tag.

(Chen et al., 2020)
Allele components
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
Mmus\Cd8a
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Mmus\Cd8asnRNA:U6:96Ac.4gRNAs
      Name Synonyms
      Secondary FlyBase IDs
        References (1)