UAS regulatory sequences drive expression of four sgRNAs; two target fz (sgRNA sequences ATGTGGCAGTCCATCTAGTTCGG and GGCATAATGGTCATGTTATATGG) and two target fz2 (sgRNA sequences ATACCAATGTGTCGGGGCATTGG and GGATCCCAATCTGCGATGCGAGG). The sgRNAs are expressed as a single polycistronic tRNA-gRNA cassette.