A E208A amino acid substitution has been introduced into the endogenous me31B locus. In addition, a sfGFP tag has been introduced at the C-terminal end of the open reading frame.
The E208A mutation was induced on the endogenouse me31B gene (which is also tagged with sfGFP). Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.