FB2026_02 , released June 18, 2026
Allele: Dmel\SarmE1170A.loxP
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General Information
Symbol
Dmel\SarmE1170A.loxP
Species
D. melanogaster
Name
FlyBase ID
FBal0398890
Feature type
allele
Associated gene
Dmel\Sarm
Associated Insertion(s)
TI{TI}SarmE1170A.loxP
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Herrmann et al., 2022)
cre recombinase
(Herrmann et al., 2022)
Progenitor genotype
TI{TI}SarmKO
(Herrmann et al., 2022)
Associated Insertion(s)
TI{TI}SarmE1170A.loxP
Cytology
Description

Genomic DNA has been inserted into the attP site in TI{TI}SarmKO, reconstituting a full-length Sarm transcription unit that encodes catalytically inactive Sarm (a key glutamic acid residue in the NADase active site has been mutated to alanine). A 50 nucleotide attR site in the intron preceding exon 17 and a 34 nucleotide loxP site in the intron succeeding exon 21/22 remain.

(Herrmann et al., 2022)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
3L:8,105,128..8,105,129 [+]
Nucleotide change:

AA8105128CC

Reported nucleotide change:

AA?CC

Amino acid change:

E1170A | Sarm-PD; E893A | Sarm-PE; E919A | Sarm-PF; E1095A | Sarm-PG; E919A | Sarm-PH; E1263A | Sarm-PI; E919A | Sarm-PJ; E1263A | Sarm-PK; E737A | Sarm-PL

Reported amino acid change:

E1170A

Comment:

Sequences containing the ARM domain, two SAM domains, and a mutated TIR domain hav been introduced into the attP site present in TI{TI}SarmKO. Sarm is catalytically inactive due to the mutation of the NADase catalytic glutamic acid residue (GAA) to an alanine (GCC)) in the TIR domain. A 50 nucleotide attR site in the intron preceding exon 17 and a 34 nucleotide loxP site in the intron succeeding exon 21/22 remain.

(Herrmann et al., 2022)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
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      Modifiers Based on Experimental Evidence ( 0 )
      Disease
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      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
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      Phenotypic Class
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      Genetic Interactions
      Statement
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      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
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      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      SarmE1170A.loxP
      dSARME1170A
      (Herrmann et al., 2022)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)