FB2026_03 , released September 17, 2026
Allele: Dmel\kniΔASE.intron.24xMS2
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General Information
Symbol
Dmel\kniΔASE.intron.24xMS2
Species
D. melanogaster
Name
FlyBase ID
FBal0403644
Feature type
allele
Associated gene
Dmel\kni
Associated Insertion(s)
TI{TI}kni-intron.24xMS2
TI{TI}kni-knrl-ΔASE
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Levo et al., 2022)
Progenitor genotype
TI{TI}kni2xattP
(Levo et al., 2022)
Associated Insertion(s)
TI{TI}kni-intron.24xMS2
TI{TI}kni-knrl-ΔASE
Cytology
Description

The genomic region of kni that is deleted in TI{TI}kni2xattP (approximately 8.9kb) has been re-integrated into the genome (by replacing the inverted attP site flanked RMCE cassette in TI{TI}kni2xattP). The re-integrated sequence has 24 copies of the Tag:MS2 stem loop inserted into the second intron. In addition, the shared kni/knrl anterior stripe enhancer (coordinates 3L:20622810..20624645 ) has been replaced by an inert sequence (derived from the y gene) of the same length.

(Levo et al., 2022)
Allele components
Component
Use(s)
Tagged with
Tag:MS2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      kniΔASE.intron.24xMS2
      Name Synonyms
      Secondary FlyBase IDs
        References (1)