UAS sequences regulate expression of a deleted form of als that lacks the PUG domain. Silent mutations have been introduced to render the transgene insensitive to RNAi mediated by M{10xUAS-als.RNAi.3-5'UTR}.
Scer\GAL4vg.PU/amlsΔPUG.3-5'UTR-RR.UAS fails to rescue amlsRNAi.10xUAS.3-5'UTR