Sequence has been re-integrated into the attP site in TI{TI}tsgattP, reconstituting a wild-type tsg locus in which the coding sequence is tagged at the C-terminal end with Tag:ALFA.
The entire tsg transcription unit has been deleted and replaced with a sequence cassette encoding 1. an attP site and 2. a loxP cassette containing a Disc\RFPDsRed(T1).3xP3.cHa marker. The deletion endpoints onthe reference sequence were inferred from the reported homology arms.