FB2026_02 , released June 18, 2026
Allele: Dmel\NFSHR.A2-R1597W.UAS.mCh.Tag:V5
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General Information
Symbol
Dmel\NFSHR.A2-R1597W.UAS.mCh.Tag:V5
Species
D. melanogaster
Name
FlyBase ID
FBal0404867
Feature type
allele
Associated gene
Dmel\N
Associated Insertion(s)
Carried in Construct
M{UAS-FSHR-mCh-A2(R1597W)-N-V5}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Langridge and Struhl, 2017)
Progenitor genotype
Carried in construct
M{UAS-FSHR-mCh-A2(R1597W)-N-V5}
Cytology
Description

UASt sequences regulate expression of N in which a. the N-terminal EGF repeat containing part of the extracellular domain has been replaced by sequence encoding a signal peptide and Tag:LBD(FSHR) and b. the juxtamembrane NRR (negative regulatory region) has been replaced with the A2 domain of the Homo sapiens VWF gene (HGNC:12726), mutated to carry a R1597W amino acid substitution. The coding sequence is tagged with mCherry just upstream of the A2 domain and with Tag:V5 in the intracellular domain. A single FRT site is present in between the UASt and coding sequences.

(Langridge and Struhl, 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
N
Tagged with
mCherry
Tag:LBD(FSHR)
Tag:V5
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      NFSHR.A2-R1597W.UAS.mCh.Tag:V5
      Name Synonyms
      Secondary FlyBase IDs
        References (1)