FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\p53RNAi.UAS.cUa
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General Information
Symbol
Dmel\p53RNAi.UAS.cUa
Species
D. melanogaster
Name
FlyBase ID
FBal0407707
Feature type
allele
Associated gene
Dmel\p53
Associated Insertion(s)
Carried in Construct
P{UAS-p53.RNAi.U}
Also Known As
UAS-p53RNAi
Key Links
Transgenic product class
RNAi_reagent
(FlyBase Curators, 2025-)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(FlyBase Curators, 2025-)
Mutagen
in vitro construct
(FlyBase Curators, 2025-)
Progenitor genotype
Carried in construct
P{UAS-p53.RNAi.U}
Cytology
Description

UAS regulatory sequences drive expression of an RNAi construct targeting p53.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
p53
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Alzheimer's disease 1
      modeled by Hsap\APPT12.Aβ42.UAS.Tag:SS(nec)
      (Taylor and Tuxworth, 2019)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression for one week during adulthood of p53VDRC.cUa under the combined control of Scer\GAL4byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) does not affect the apoptosis levels in the hindgut, does not lead to the dissemination of hindgut cells through the body and does not affect the replication of cells (i.e. BrU-positive) in the pylorus, as compared to controls.

      (Bangi et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Statement
      Reference

      ApcRNAi.UAS.cUa, MedRNAi.UAS.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has short lived phenotype

      (Bangi et al., 2016)

      ApcRNAi.UAS.cUa, MedRNAi.UAS.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has hyperplasia | adult stage phenotype

      (Bangi et al., 2016)

      MedRNAi.UAS.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has abnormal cell shape | adult stage phenotype

      (Bangi et al., 2016)

      ApcRNAi.UAS.cUa, MedRNAi.UAS.cUa, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has hyperplasia | adult stage phenotype

      (Bangi et al., 2016)

      ApcRNAi.UAS.cUa, MedRNAi.UAS.cUa, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has neoplasia phenotype

      (Bangi et al., 2016)

      ApcRNAi.UAS.cUa, MedRNAi.UAS.cUa, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has abnormal cell shape | adult stage phenotype

      (Bangi et al., 2016)

      ApcRNAi.UAS.cUa, MedRNAi.UAS.cUa, PtenGD13500, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has hyperplasia | adult stage phenotype

      (Bangi et al., 2016)

      ApcRNAi.UAS.cUa, MedRNAi.UAS.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has neoplasia phenotype

      (Bangi et al., 2016)

      ApcRNAi.UAS.cUa, MedRNAi.UAS.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has abnormal cell shape | adult stage phenotype

      (Bangi et al., 2016)

      ApcRNAi.UAS.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has hyperplasia | adult stage phenotype

      (Bangi et al., 2016)

      ApcRNAi.UAS.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has neoplasia phenotype

      (Bangi et al., 2016)

      ApcRNAi.UAS.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has abnormal cell shape | adult stage phenotype

      (Bangi et al., 2016)

      MedRNAi.UAS.cUa, PtenGD13500, Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa has hyperplasia | adult stage phenotype

      (Bangi et al., 2016)
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Statement
      Reference

      p53RNAi.UAS.cUa, MedRNAi.UAS.cUa, ApcRNAi.UAS.cUa, PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa, MedRNAi.UAS.cUa, ApcRNAi.UAS.cUa is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa, MedRNAi.UAS.cUa, ApcRNAi.UAS.cUa is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      p53RNAi.UAS.cUa, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      p53RNAi.UAS.cUa, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      p53RNAi.UAS.cUa/PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      p53RNAi.UAS.cUa/PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      p53RNAi.UAS.cUa, MedRNAi.UAS.cUa, ApcRNAi.UAS.cUa, PtenGD13500, Scer\GAL4byn-Gal4 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa, ApcRNAi.UAS.cUa, PtenGD13500 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa, ApcRNAi.UAS.cUa, PtenGD13500 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa, MedRNAi.UAS.cUa, PtenGD13500 is an enhancer of adult hindgut phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)

      Scer\GAL4byn-Gal4, p53RNAi.UAS.cUa, MedRNAi.UAS.cUa, PtenGD13500 is an enhancer of adult hindgut epithelium phenotype of Ras85DV12.cUa.UAS, Scer\GAL4byn-Gal4

      (Bangi et al., 2016)
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Quadruple or quintuple co-expression for one week during adulthood of Ras85DV12.cUa.UAS, p53VDRC.cUa, PtenGD13500, ApcVDRC.cUa and/or MedVDRC.cUa under the combined control of Scer\GAL4byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) leads to multilayered epithelia that form bulges at discrete points along the hindgut (only in animals that contained Ras85DV12.cUa.UAS and ApcVDRC.cUa together) and to an expanded pylorus (observed in the quintuple and all quadruple conditions that carried Ras85DV12.cUa.UAS); the quadruple co-expression conditions Ras85DV12.cUa.UAS+p53VDRC.cUa+PtenGD13500+MedVDRC.cUa and p53VDRC.cUa+PtenGD13500+ApcVDRC.cUa+MedVDRC.cUa do not lead to multilayering.

      All quadruple and quintuple co-expression conditions - with the exception of the quadruple p53VDRC.cUa+PtenGD13500+MedVDRC.cUa+ApcVDRC.cUa - result in numerous hindgut cells losing their characteristic epithelial shape to assume a more mesenchymal appearance (processes extending towards the basement membrane and surrounding muscle layer), migrate locally and disseminate to distant sites in the organism (e.g. head, legs, fat body, ovaries, below the epidermis of the abdomen and within the abdominal cavity). Similar hindgut cell dissemination is observed upon the triple expressions of Ras85DV12.cUa.UAS+p53VDRC.cUa+PtenGD13500, Ras85DV12.cUa.UAS+ApcVDRC.cUa+PtenGD13500 or Ras85DV12.cUa.UAS+p53VDRC.cUa+ApcVDRC.cUa, the double expressions of Ras85DV12.cUa.UAS+p53VDRC.cUa, Ras85DV12.cUa.UAS+PtenGD13500 or Ras85DV12.cUa.UAS+ApcVDRC.cUa, all of which are more severe than upon the single expression of Ras85DV12.cUa.UAS alone.

      The expression for one week during adulthood of p53VDRC.cUa, alone or together with PtenGD13500 or ApcVDRC.cUa, under the combined control of Scer\GAL4byn-Gal4, Dicer-2 (for efficient RNAi) and Gal80[ts] (for the temporal control of expression) does not affect the replication of cells (i.e. BrU-positive) in the pylorus, as compared to controls.

      The quadruple co-expression condition p53VDRC.cUa+PtenGD13500+MedVDRC.cUa+ApcVDRC.cUa leads to a very mild cell dissemination through the body.

      (Bangi et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      p53RNAi.UAS.cUa/Scer\GAL4sd.PU partially rescues p53UAS.DΔNp53

      (Simón et al., 2014)
      Comments

      Additional expression of p53TRiP.cUa mostly rescues the adult wing morphological defects caused by expression of p53UAS.DΔNp53 under the control of Scer\GAL4sd.PU.

      (Simón et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      p53RNAi.UAS.cUa
      p53TRiP.cUa
      p53VDRC.cUa
      p53dsRNA.Scer\UAS.cUa
      p53dsRNA.UAS.cUa
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0316561
      • FBal0344238
      • FBal0342088
      References (9)