In individuals expressing spag^VDRC.cUa under the control of Scer\GAL4^VP16.bam, testes lack motile sperm and, although sperm bundles are largely normal, axonemes show loss of dynein arms, occasional microtubule occlusions and axonemal fragmentation.

In individuals expressing spag^VDRC.cUa under the control of Scer\GAL4^sca.PU, Ch neurons show a grossly normal cilium morphology despite of the absence of dynein arms.

Expression of spag^{dsRNA.Scer\UAS.cUa} in clock cells under the control of Scer\GAL4^tim.PE leads to altered locomotor activity rhythms relative to wild-type. The spag^{dsRNA.Scer\UAS.cUa}-expressing flies exhibit long periods (5.5-26.5 h) or arrhythmic locomotor activity depending on the specific RNAi transgene, the inclusion of Dcr-2 in the genotype and the duration of transgene expression after eclosion.

Flies expressing Scer\GAL4^tim.PE>spag^{dsRNA.Scer\UAS.cUa} show circadian-dependent activation of apoptosis in the head at an earlier age than do wild-type flies.

Flies expressing Scer\GAL4^tim.PE>spag^{dsRNA.Scer\UAS.cUa} initially climb as well as control flies. However, over time they decline in performance more rapidly than controls. In addition, the Scer\GAL4^tim.PE>spag^{dsRNA.Scer\UAS.cUa} flies show reduced lifespan compared with controls.