FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\tkv3xHA
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General Information
Symbol
Dmel\tkv3xHA
Species
D. melanogaster
Name
FlyBase ID
FBal0407962
Feature type
allele
Associated gene
Dmel\tkv
Associated Insertion(s)
TI{TI}tkv3xHA
Carried in Construct
Also Known As
Tkv-3xHA
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
cre recombinase
(Cai et al., 2019)
phiC31 integrase
(Cai et al., 2019, Norman et al., 2016)
Progenitor genotype
tkvKO.attP
(Cai et al., 2019)
Associated Insertion(s)
TI{TI}tkv3xHA
Cytology
Description

Three copies of the Tag:HA epitope have been introduced at the C-terminal end of the coding sequence (CDS) in the endogenous tkv locus. This allele was generated in a multi-step process that comprised: 1. insertion of sequence containing the last two exons of tkv (and intervening intron), modified to include three copies of the Tag:HA epitope at the C-terminal end of the CDS, into the attP site present in tkvKO.attP, 2. removal of a loxP cassette containing a w+* selectable marker. An attP/B hybrid sequence is present in the last large intron and a single loxP site is also present in the 3'UTR as a remnant of this genome engineering process.

(Cai et al., 2019)

Three copies of the Tag:HA epitope have been inserted into the endogenous tkv locus, immediately before the stop codon.

(Norman et al., 2016)
Allele components
Component
Use(s)
Tagged with
Tag:HA
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      tkv3xHA homozygotes develop normally, display no obvious phenotypic abnormality and are fully fertile.

      (Cai et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TkvHA
      (Norman et al., 2016)
      tkv3xHA
      tkvHA
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0355580
      • FBal0367998
      References (4)