FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Cirl3x-tagged.H-A
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General Information
Symbol
Dmel\Cirl3x-tagged.H-A
Species
D. melanogaster
Name
FlyBase ID
FBal0408146
Feature type
allele
Associated gene
Dmel\Cirl
Associated Insertion(s)
TI{TI}Cirl3x-tagged.H-A
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Bormann et al., 2025)
Progenitor genotype
TI{TI}CirlKO.attP.w-
(Bormann et al., 2025)
Associated Insertion(s)
TI{TI}Cirl3x-tagged.H-A
Cytology
Description

Genomic sequence has been inserted into the attP site in TI{TI}CirlKO.attP.w-, reconstituting a Cirl locus in which a H>A mutation has been introduced into the GPCR proteolysis site (GPS) to abolish autoproteolytic cleavage. The coding sequence is tagged in 3 different locations. A mRFP(Unk) tag is inserted within the N-terminal fragment of the extracellular region and tags both the Cirl-1TM and Cirl-7TM protein isoforms. In addition, the Cirl-1TM protein isoform is tagged with 2xTag:V5 at the C-terminal end of the coding sequence and the Cirl-7TM protein isoform is tagged with 3xTag:FLAG in the third intracellular loop.

(Bormann et al., 2025)
Allele components
Component
Use(s)
Tagged with
mRFP(Unk)
Tag:FLAG
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Cirl3x-tagged-GPS/H>A
      (Bormann et al., 2025)
      Cirl3x-tagged.H-A
      Name Synonyms
      Secondary FlyBase IDs
        References (1)