FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\RAC1Rac-FRET.UAS
Open Close
General Information
Symbol
Hsap\RAC1Rac-FRET.UAS
Species
H. sapiens
Name
FlyBase ID
FBal0410810
Feature type
allele
Associated gene
Hsap\RAC1
Associated Insertion(s)
Carried in Construct
P{UAS-YPet-PAK-RAC-CFP.FRET}
Also Known As
UAS-RacFRET, UAS-Rac-FRET
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Wang et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-YPet-PAK-RAC-CFP.FRET}
Cytology
Description

UAS regulatory sequences drive expression of a fluorescent biosensor for Rac activity. The biosensor is composed of two fluorescent proteins (YPet and CFP) which are separated by sequence encoding the Cdc42- and Rac-interactive binding motif of PAK1 followed by the Hsap\RAC1 coding region. When the Hsap\RAC1 polypeptide is activated (by binding GTP instead of GDP), it is bound by the PAK1 motif, altering the conformation of the protein and increasing the fluorescence resonance energy transfer (FRET) between the two fluorophores.

(Wang et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\RAC1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\RAC1Rac-FRET.UAS
      Uuuu\Rac1Rac-FRET.UAS
      Uuuu\Rac1Scer\UAS.T:Avic\GFP-YPet,T:Uuuu\PAK,T:Avic\GFP-CFP
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0410733
      • FBal0243925
      References (9)