FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\Nfly-060
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General Information
Symbol
Dmel\Nfly-060
Species
D. melanogaster
Name
Nfly-060
FlyBase ID
FBcl0006235
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG15394
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    CB_cDNA
    • CB cDNA library of D. melanogaster, iso-1 strain, mixed stages.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism
      (Lee et al., 2005)
      second instar larval stage
      organism
      (Lee et al., 2005)
      third instar larval stage
      organism
      (Lee et al., 2005)
      pupal stage
      organism
      (Lee et al., 2005)
      adult stage | male
      testis
      (Lee et al., 2005)
      adult stage
      organism
      (Lee et al., 2005)
      Sequence Data of the Insert
      Partial Sequence
      Total bases
      388
      NCBI
      CB305245
      Partial sequence dataSequence Downloader
      AGAAACTATTCAGAGTGAAGAGGGCATCCAGAGCACTTGCAGCTCAAAAAATAAGCGGTTTTTCCCACTCCTCACCACTT
TTAGGCTGACCACAAGTTCCGGAAGATTCAGCTCCTCAAGTAGTGCCTTTTTTAGGCTCTGTAGAGGATCATCCACTTTC
TTGTAGCTGATGAAGATCAGGCGAGCATTGCCAGAAGCGTTGTCCAGCTGGAAGTCAGTGCATTTGGCCAGTTTCAAGTC
TCTGGACTGCGATGAGTGTTCATTTGAGTACTGCTGCCAGGGGCGAATGACTTGCTCATCTTCGGTGGGGAATACAAAGT
CATTTACGTACGAGGTCTTTGCCCTGGGATAGGCGAAACAACTGCCAAGGCAAATAAGAGCCCACATG
      Library Information (1)
      Library: CB_cDNA
      Description

      Partial cDNAs generated with the GLGI technique; extend from the 3' poly(A) tail to the first CATG.

      (Lee et al., 2005)
      Sample preparation

      A combination of embryos, second and third larvae, third day pupae, and young and aged adults, irradiated embryos and testis were used.

      Protocol

      Four SAGE libraries were constructed: (1) pooled sample including equal amounts of total RNA from embryos, second and third larvae, third day pupae, and young and aged adults, (2) embryos, (3) irradiated embryos and (4) testis. Unmatched (to annotated transcripts) SAGE tags from the four libraries were converted into 3' cDNAs using the GLGI (generation of longer 3' cDNA from SAGE tag for gene identification) method. Sequences extend from the poly(A) tail to the first CATG site.

      (Lee et al., 2005)
      Comment

      GenBank CB305186-CB305318.

      (Lee et al., 2005)
      Reported As
      Symbol Synonyms
      CB
      CB_cDNA
      References (1)
      Research paper (1)
      Lee et al., 2005, RNA 11(6): 939--946
      Detecting novel low-abundant transcripts in Drosophila. [FBrf0188306]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)