FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Clone: Dmel\AT12326
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General Information
Symbol
Dmel\AT12326
Species
D. melanogaster
Name
AT12326
FlyBase ID
FBcl0014451
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG16711
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      testis
      (Stapleton et al., 2002)
      seminal vesicle
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      4,989
      NCBI
      BT001300
      Full Length sequence dataSequence Downloader
      AAAAAAACGAGCGACGAGTGTGGACTAGCCAGCGATGATGGGAAAATGACCAGGGATCTGAGAGTACGGATCACGGACCA
CTAGCGGATACGGCCGTAAAATGCCCCGGAACACTTGCAGGCCAAGTGAATGCGCTGCTGCTAGCCAGGATGAGCCGGAG
GCGAGTGCGAATAACAACAATAACAACAGCAACAACAACAACAATAACGAGGGCATCATCAAGCAGGAACTGGAGGCCGC
TCCGCCGGTTTGCCACCGAAAATCGGAGCGCCGGAGAGTGGCCCGTGATATATTCCTGGTCTTCGAGGAGCAGACCACCC
GGCGACGAGGGAAGGGAGCACGGGGCAAGGCCACAAGGGCAAAGCAAAAGTTTGGACACCTGAAGGTGGCCAATACGCCG
CGCATCAATAAGAATGTGCTGCGCGACATTAAAACGGAACCAAAGTGCAGCTCGGAAGCCGTTGTGCCGGCTGCTGCCCC
AGCTATCATACCCATTCCCGCACTGAATCCCCCAATTTCTCCGCAAATAGCCGCGCTCAATCCTCCGTTGCCGGAGGAGC
CGACGTTAGCCAAATCCTTGCCGCGAACGGCGGCCGTAAAATTGGAAACCAAAACGCACGTCACATCCACAGCCACTGCA
TCCAATGTGGCGGCACCCAAGCGAATTAGTCCCAAGCCCAAGGCTCCATCTGTTCAGGAGAAGGAAAAGGAGGTGGTGTC
AGCGGTCACTCCTGTTTCTCTACCAATACCCAAGCCCGCTGCCGCACCCGCCACTTCGCCCACGACGCCCAGCGTCAATC
CGATCTTCCTGTGGGTCAAACAGGACGATACCCGCATCGTGGAGGTGCGCTGCGAGGACTACGACAAGCGGAATAGGATT
CGTCTGACCAAGACGACCAATGGCTGGCGATCTATGCCGCGCACAGATGCTTCCTCAACTAGGATAGTCAAATTCTTCCA
TGGCTCCGCCGCCCCACTGATGAAGGTCAAGCGCGAGCAGCATCTGCAGCGTTTGGAGGAGGAGGAGCAACAGCACCAGG
AGCAGGAGTTGGAACAGGCGGAGGATCATGAGCGAGAGCAGGAGCAGTCGGAGCAGCAGATGCCCTCCATCGGGCAAACT
TTGTTGCAGGATTGGGATGATAGTGCCACGCAATTGCCGGACGAGATCGAAGAGCAGGAGGAGGAGGAAGAGAGACAGGA
AAGTGATGCTGCGCAGCAGCAGAACGGCCACTTAGCCACTGTACTCGAAGATGTTGTGGTGATGCCACCTGCCCAAGAGC
CGGAGATGGCGCCCACGCCCTGCTTCGATGGCCTGCTGGACAGCGAGGAGACGAATGCCTCCACCAAGGCCACCAAGACC
AATACGCCCACATATCAGAGCTCCACGCCCTCGCCGCTGCAAATGCAGCTGTGTCCCAAGACGGGTCTGTTTCTGCCGAA
AGGAGATCTTGCCCTGGTTCAGCTGCCTGAGGAGGATGTCCTGCCCGAGGAGGCGGAGGACAAGCCACTGGGTGGAAACG
ATGCCGTGGAGTGTTTCGATGCGAATACTAAGAATCTGAAGGATCTGCTGGACGATGCCGATGATCTGCTACAGAATTGT
AGCAGTGAAAACGGCACCAGTGGAGCGGATTTGCTCGACTCGCTGGTCAAGCGAAGTTGCGAGGACAATCTCGTCCAGGA
GACGCGCCCTGGCAACGGAACCAATCCGCCGAACTCCTCAGCCTCCGGCGACGGACTGAGCTCGTTTTGTGTACCCGATC
TCGACAAGGACCTGCCCCGCATACTCAACATGGACAACGACGATGCGCCCAAGTGTCTGTCCTTCAATGAGGCGGGTGAG
ATCGAGGGCCTGAACGGAGAGCTCTTCCTGGGCATCGAGGCGTTCGAGAAGCAGCATGAAGCTGTGGCCACCGAGCCGGA
ACCACAACCGCCCACTCCAGCCACTGTGGAAATGACCCAAACGCCTACGCCGACACCGACGCCTGAAAAGGAAATGGAAA
AATCCGCCGTAGTCGAGGAAACGCCCAAGGATTTGAGCTACAAGAAACGCGAGGAGGTCTGCCCACCATCAGAATCGCGT
AGTCAGTGTGCGGTGACCTCAAGTTCGGATATACTCAAGTCACCGGAGCGGGAACTCCAGCTGCCGGCCAACTCCATACC
TGCTGAGGTGGAGACGACGTCGGAAACCATTAAAAATCTCATCCTGGAGCAGTTCCTCAAGCTTAACAACAATCCCCAAC
AGGCAGAGCCCATGGATCTGGGCAAAGCTGGCCGCGATGGCAAACTCGAAACGGTCGTTATCGACGACGAGGAGGAGGAG
GATTCCAGCCAGCCGCTGAAGAAGCGACCCAAGGCCAGCATTAAGATGGACAAGGATCCGGATCCACTTACCCAACTGAA
AATGCTGATCAGCAATCCGCAGTGGAAGGTCCCAGACCCCATACTCGTGCCAAAGGATCGCCTCGGAGCGGTTTTGGCCT
CACCGGCCCGAGAGATTCCATTGCTGCTGACAACCAGGCCGGAGTTGCGTCTTCCCGAGGCCTTTGCCTATCCGGAGATC
ATCCAGAATCCGAACATTCTGGTAGTGACCATGGAGCAGTTGGAGGCCATCCTCAAAACGGAATCGGAGCAGGCGGCCAA
GGTGACAAAGGACCCGGATCCAGTGATCGTGGTGCCAAGCAAAGCGAGAAGTCCCAGTCCCAAGCAGATACAACCCAAAC
CTGTGCCTGTTCAACCCAAACCGCCTGCAGTTGCCCCAGTGGCACCAACACCACCATCTCCGGCAGATTCCAGTCTCTCC
ACGGATCTGAATGCCACCACACTGGCGCTACTCCAACAGATGCTGTGGCTACCATATTTTGGCCAGTTGTCGCAGGAGTT
CTTCAAAACGATCAAAGATCCGCTGGGACTACAAAGAAAGTTTATGAGCAATCTCCTGCCGCTGTACAACAACCAATTTG
GATTGCAGCATTTGGACGAGCTGTACAAGCATTGTATGAAACAAAAGTCGACGGAAGAGCAGCCACAGCCCAAGGTTACC
ACTCCCGTGCCGCCTCCTGCAGCAGCTCCGCCTCCACCTCTTCCTCAAAGTACAGCCAGCGGACCAAATTCCTTTAATGG
CTTCACCAATCCCGTAGAGCTGGCTCTCATGCAAAAGCTGCTGCAGCCGCAATTGCCACAGTTCCTCAACTGGGAGGCGG
CCAAGGAGGCATCATCCACATCATCGGCAGCTGCCACCTCAAATCATCATCATCACCAGGAGCACAAGCGACCCAGAAGG
GAAACAGAAACGGAATCCATTAGCAATTTCACCAAATCCAAGCCTTCATCCGGTGCTCCAACTACAACAGCTGCTCCACC
ACCAGCCGCCGCTGCGCCTGTGCCGGTGGCCACCGCTGCGCCTCCGGCACCAGCTCCGGCGGAGCACAAACCCAGACTGA
CCATTAAATCCCTCTCGAATCTGCTGGAACCGGAGGCCAACGTGGTTCCACTACTCAACGTGCCCTTCGACGGAATGCGA
GGGCGTCCCTCCATGCACAAGGCCGTTCCAGATCAGGCCAAGGGTGACGCAGGTACGGGACGCACACTACGCTCCAGTAA
AGCCTGCGTCACCTATAATCCCCTCGAGCAGGCCAAGCAGCAAATGCACCCAGCCGCCGCCGCACAGCAGCAGCGCAAGC
GGGGCAATATGCTCGCCCAGGACGTGCAGCAGCAACAGCACCAGCAAAATCTTGCAGATGCGGCGGCAGCAGCCGGCATG
GATGCCAACTCGGCACTGTGGCATCCTCTCTTCGGAAGCAATCCCAAACAAGGCTACAATAGTCCATGGCAGTGGACCAC
GGTGACGGCATCAGGTGAATGACAGTCAAGCCGGGCTCAAAAGGAGCCAGCACCTGGATCGGGAACAAGGACGGCCAGCT
ACGAGGAGCAGCATCAGCAGCAGGACTACAGCAACTATCACGCCTCGGCCAAGCAACAGCAGCAACAGCAACCACAACTT
CCAGCAGCCAACAATCAAAACCACAGCAACAATAGTAGCAATAAATTTTGTTATCCAACGGCAGCGGCCATGGAGAATAT
GTTCCAGAACAATCAGATGGCGGCCGCTGCCGCCGCACGCGAATCCCTGCTGAATTCATATCTCTATCAAAAGGATAGCG
ATGCCGGCAATTATATGGCGGAGCAGAGCTACTGGCAACAGCACCAGCAACATCACCACCAACAGCAGCAGCAGCAGCAG
CATCAACAACAACAGCAGCAGCAACATCAACAGCAGCAACATCAACAGCAGCAGCAACACCAACAACAGCAACACCAACA
GCAGCAACAGCAACAGAAGCAGCTCCAGCAGCAGCAGCAGCAGCATCAATGGAGCGCCAACAACGGCAGCAGCAACCAAA
GGAGCAACAATTATGCCAACAACTATGCGGCGGCAGCCGCTGCCTCCATGTACATGCAACACTTCAATCCCTACATGCAA
CAGAAGGCGGCGCTGCTGGCGGAGAAGGCCGCCCAAGCGGCAGCGGCGGCGGCAGCGGTCCAGAGCAGTAACGGTAGCAA
TAAGCACGGACGCTTCGGCGGTGATTACGGCTAGGAGTCGTCCGGTGGGTTCACGGTTCAGCTAACCGTAGCGCAAGACA
CTTTAGCGCATTAACATAAACATATAGGCATATGTAACACTTGCGTGTAATAATAACTATATATTTAGAGGCATCGTATA
GGCCGTTTTACCGCCAGGAATTGGTAAAGGATAAACCATGATTTTCCTTTAGTTTAATACACAGGAACAGCAACAACAAG
GTAACCGGAACGCAAACAACAATAATTTATAAACAACAAACAAGGTGGTAAACACAAGCAGAGAGCAGGAAACATAAAAA
AAAACTTAAATTGAATTAGTAAAAATTTGTTATTGGTAGTTTGTTAACAAAAAAGAAAAACACAAAAAATATGAAAAGAA
AACAAAGAGGAAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)